Functional interrogation of primary human T cells via CRISPR genetic editing

Developing precise and efficient gene editing approaches using CRISPR in primary human T cell subsets would provide an effective tool in decoding their functions. Towards this goal, we utilized lentiviral CRISPR/Cas9 systems to transduce primary human T cells to stably express the Cas9 gene and guid...

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Detalhes bibliográficos
Publicado no:J Immunol
Main Authors: Chen, Xin, Kozhaya, Lina, Tastan, Cihan, Placek, Lindsey, Dogan, Mikail, Horne, Meghan, Abblett, Rebecca, Karhan, Ece, Vaeth, Martin, Feske, Stefan, Unutmaz, Derya
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6103902/
https://ncbi.nlm.nih.gov/pubmed/30021769
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4049/jimmunol.1701616
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