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Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

This video presents the use of transmission electron microscopy with energy dispersive X-ray microanalysis (TEM-EDX) to compare the state of minerals in vesicles released by two human bone cell lines: hFOB 1.19 and Saos-2. These cell lines, after treatment with ascorbic acid (AA) and β-glycerophosph...

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Bibliografski detalji
Izdano u:J Vis Exp
Glavni autori: Bozycki, Lukasz, Komiazyk, Magdalena, Mebarek, Saida, Buchet, Rene, Pikula, Slawomir, Strzelecka-Kiliszek, Agnieszka
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2018
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101988/
https://ncbi.nlm.nih.gov/pubmed/29985356
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57423
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