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CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

Brain malformation is often caused by genetic mutations. Deciphering the mutations in patient-derived tissues has identified potential causative factors of the diseases. To validate the contribution of a dysfunction of the mutated genes to disease development, the generation of animal models carryin...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Feng, Weijun, Herbst, Lena, Lichter, Peter, Pfister, Stefan M., Liu, Hai-Kun, Kawauchi, Daisuke
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101645/
https://ncbi.nlm.nih.gov/pubmed/29939173
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57311
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