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Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Living organisms regularly need to cope with fluctuating environments during their life cycle, including changes in temperature, pH, the accumulation of reactive oxygen species, and more. These fluctuations can lead to a widespread protein unfolding, aggregation, and cell death. Therefore, cells hav...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Fassler, Rosi, Edinger, Nufar, Rimon, Oded, Reichmann, Dana
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101639/
https://ncbi.nlm.nih.gov/pubmed/29939186
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57806
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