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Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Living organisms regularly need to cope with fluctuating environments during their life cycle, including changes in temperature, pH, the accumulation of reactive oxygen species, and more. These fluctuations can lead to a widespread protein unfolding, aggregation, and cell death. Therefore, cells hav...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Fassler, Rosi, Edinger, Nufar, Rimon, Oded, Reichmann, Dana
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101639/
https://ncbi.nlm.nih.gov/pubmed/29939186
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57806
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