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Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

We present a method of analyzing sphingomyelin (SM) qualitatively and quantitatively by liquid chromatography-electrospray Ionization-tandem mass spectrometry (LC-ESI-MS/MS). SM is a common sphingolipid composed of a phosphorylcholine and a ceramide as the hydrophilic and hydrophobic component, resp...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Hama, Kotaro, Fujiwara, Yuko, Yokoyama, Kazuaki
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101123/
https://ncbi.nlm.nih.gov/pubmed/29782002
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57293
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