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Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

The western corn rootworm (WCR) is an important pest of corn and is well known for its ability to rapidly adapt to pest management strategies. Although RNA interference (RNAi) has proved to be a powerful tool for studying WCR biology, it has its limitations. Specifically, RNAi itself is transient (i...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Chu, Fu-Chyun, Wu, Pei-Shan, Pinzi, Sofia, Grubbs, Nathaniel, Lorenzen, Marcé D.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101034/
https://ncbi.nlm.nih.gov/pubmed/29757280
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57497
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