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Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
To complement existing affinity purification (AP) approaches for the identification of protein-protein interactions (PPI), enzymes have been introduced that allow the proximity-dependent labeling of proteins in living cells. One such enzyme, BirA* (used in the BioID approach), mediates the biotinyla...
Guardat en:
| Publicat a: | J Vis Exp |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
MyJove Corporation
2018
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6100705/ https://ncbi.nlm.nih.gov/pubmed/29733317 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57479 |
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