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Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates
In an effort to increase efficiency in the creation of genetically modified mice via ES Cell methodologies, we present an adaptation to the current blastocyst injection protocol. Here we report that a simple rotation of the embryo, and injection through Trans-Inner cell mass (TICM) increased the per...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6085124/ https://ncbi.nlm.nih.gov/pubmed/29912196 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56955 |
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