A Novel qPCR Method for Simultaneous Detection and Quantification of Viable Pathogenic and Non-pathogenic Vibrio parahaemolyticus (tlh(+), tdh(+), and ureR(+))

Pathogenic and non-pathogenic Vibrio parahaemolyticus strains were simultaneously detected and quantified using a novel viable multiplex real-time PCR (novel qPCR). We used a new PCR primer and probe, ureR, as a surrogate for detection of the toxin trh gene as the primer was better at identifying va...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Niu, Ben, Hong, Bin, Zhang, Zhaohuan, Mu, Lili, Malakar, Pradeep K., Liu, Haiquan, Pan, Yingjie, Zhao, Yong
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2018
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6083054/
https://ncbi.nlm.nih.gov/pubmed/30116230
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2018.01747
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