Expression, purification, and characterization of biologically active full-length Mason-Pfizer monkey virus (MPMV) Pr78(Gag)

MPMV precursor polypeptide Pr78(Gag) orchestrates assembly and packaging of genomic RNA (gRNA) into virus particles. Therefore, we have expressed recombinant full-length Pr78(Gag) either with or without His(6)-tag in bacterial as well as eukaryotic cultures and purified the recombinant protein from...

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Veröffentlicht in:Sci Rep
Hauptverfasser: Pitchai, Fathima Nuzra Nagoor, Ali, Lizna, Pillai, Vineeta Narayana, Chameettachal, Akhil, Ashraf, Syed Salman, Mustafa, Farah, Marquet, Roland, Rizvi, Tahir Aziz
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2018
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6081465/
https://ncbi.nlm.nih.gov/pubmed/30087395
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-30142-0
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