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Metabolic engineering using iterative self-cloning to improve lipid productivity in Coccomyxa

We previously developed a self-cloning system that introduces cDNA of the uridine monophosphate synthase gene (cUMPS) of Coccomyxa sp. strain Obi as a selectable marker into uracil-auxotrophic mutants (Ura(−)) of the same alga. Here, we developed a Cre/loxP-based system for the removal of cUMPS flan...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Kasai, Yuki, Tsukahara, Takuya, Ikeda, Fukiko, Ide, Yoko, Harayama, Shigeaki
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6078956/
https://ncbi.nlm.nih.gov/pubmed/30082815
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-30254-7
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