High doses of CRISPR/Cas9 ribonucleoprotein efficiently induce gene knockout with low mosaicism in the hydrozoan Clytia hemisphaerica through microhomology-mediated deletion

Targeted mutagenesis using CRISPR/Cas9 technology has been shown to be a powerful approach to examine gene function in diverse metazoan species. One common drawback is that mixed genotypes, and thus variable phenotypes, arise in the F0 generation because incorrect DNA repair produces different mutat...

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Veröffentlicht in:Sci Rep
Hauptverfasser: Momose, Tsuyoshi, De Cian, Anne, Shiba, Kogiku, Inaba, Kazuo, Giovannangeli, Carine, Concordet, Jean-Paul
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2018
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6078951/
https://ncbi.nlm.nih.gov/pubmed/30082705
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-30188-0
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