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Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes

The architecture of normal and diseased tissues strongly influences the development and progression of disease as well as responsiveness and resistance to therapy. We describe a tissue-based cyclic immunofluorescence (t-CyCIF) method for highly multiplexed immuno-fluorescence imaging of formalin-fix...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Lin, Jia-Ren, Izar, Benjamin, Wang, Shu, Yapp, Clarence, Mei, Shaolin, Shah, Parin M, Santagata, Sandro, Sorger, Peter K
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6075866/
https://ncbi.nlm.nih.gov/pubmed/29993362
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.31657
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