One-phase phenol-free method for microRNA isolation from blood plasma

MicroRNA extraction is an essential procedure when discovering MicroRNA-based biomarkers and approaches. Here we describe a new method for microRNA isolation from human blood plasma, based on isopropanol precipitation from the one-phase lysate. We demonstrate that the use of more than four volumes o...

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Bibliographic Details
Published in:MethodsX
Main Authors: Shirshova, A.N., Shamovskaya, D.A., Boyarskikh, U.A., Kushlinskii, N.E., Filipenko, M.L.
Format: Artigo
Language:Inglês
Published: Elsevier 2018
Subjects:
Biochemistry, Genetics and Molecular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6070682/
https://ncbi.nlm.nih.gov/pubmed/30094203
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2018.07.002
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