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Detection of E2F-DNA Complexes Using Chromatin Immunoprecipitation Assays
Chromatin immunoprecipitation (ChIP), originally developed by John T. Lis and David Gilmour in 1984, has been useful to detect DNA sequences where protein(s) of interest bind. ChIP is comprised of several steps: (1) cross-linking of proteins to target DNA sequences, (2) breaking genomic DNA into 300...
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| Published in: | Methods Mol Biol |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6070307/ https://ncbi.nlm.nih.gov/pubmed/29468550 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7565-5_13 |
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