Development and validation of a modified TaqMan based real-time PCR assay targeting the lipl32 gene for detection of pathogenic Leptospira in canine urine samples

A modified TaqMan real-time polymerase chain reaction targeting a 138 bp fragment within the lipl32 gene was developed to identify exclusively pathogenic Leptospira spp. in dog urine samples. Thirty-five samples from dogs with suspected clinical leptospirosis and 116 samples from apparently healthy...

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Detalhes bibliográficos
Publicado no:Braz J Microbiol
Main Authors: Miotto, Bruno Alonso, da Hora, Aline Santana, Taniwaki, Sueli Akemi, Brandão, Paulo Eduardo, Heinemann, Marcos Bryan, Hagiwara, Mitika Kuribayashi
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2017
Assuntos:
Veterinary Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6066729/
https://ncbi.nlm.nih.gov/pubmed/29233483
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bjm.2017.09.004
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