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Designing fluorescent biosensors using circular permutations of riboswitches
RNA-based fluorescent (RBF) biosensors have been applied to detect a variety of metabolites in vitro and in live cells. They are designed by combining the ligand sensing domain of natural riboswitches with in vitro selected fluorogenic aptamers. Different biosensor topologies have been developed to...
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| Publicado no: | Methods |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6051913/ https://ncbi.nlm.nih.gov/pubmed/29458090 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2018.02.014 |
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