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‘Read–through marking’ reveals differential nucleotide composition of read-through and truncated cDNAs in iCLIP
We established a modified iCLIP protocol, called ‘read-through marking’, which facilitates the detection of cDNAs that have not been truncated upon encountering the RNA–peptide complex during reverse transcription (read-through cDNAs). A large proportion of these cDNAs would be undesirable in an iCL...
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| Izdano u: | Wellcome Open Res |
|---|---|
| Glavni autori: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
F1000 Research Limited
2018
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6051193/ https://ncbi.nlm.nih.gov/pubmed/30057947 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12688/wellcomeopenres.14663.1 |
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