Assessing the tolerance to room temperature and viability of freeze-dried mice spermatozoa over long-term storage at room temperature under vacuum

Freeze-drying has been frequently used to preserve food and microorganisms at room temperature (RT) for extended periods of time; however, its application to mammalian species is difficult. Here, we developed a method to prolong the stability of freeze-dried (FD) mice spermatozoa at RT for more than...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Kamada, Yuko, Wakayama, Sayaka, Shibasaki, Ikue, Ito, Daiyu, Kamimura, Satoshi, Ooga, Masatoshi, Wakayama, Teruhiko
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2018
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6045625/
https://ncbi.nlm.nih.gov/pubmed/30006561
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-28896-8
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