Expression, Purification, and Biochemical Characterization of the Flavocytochrome P450 CYP505A30 from Myceliophthora thermophila

[Image: see text] The cytochrome P450/P450 reductase fusion enzyme CYP505A30 from the thermophilic fungus Myceliophthora thermophila and its heme (P450) domain were expressed in Escherichia coli and purified using affinity, ion exchange, and size exclusion chromatography. CYP505A30 binds straight ch...

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Udgivet i:ACS Omega
Main Authors: Baker, George J., Girvan, Hazel M., Matthews, Sarah, McLean, Kirsty J., Golovanova, Marina, Waltham, Timothy N., Rigby, Stephen E. J., Nelson, David R., Blankley, Richard T., Munro, Andrew W.
Format: Artigo
Sprog:Inglês
Udgivet: American Chemical Society 2017
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6044835/
https://ncbi.nlm.nih.gov/pubmed/30023729
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsomega.7b00450
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