Enabling Biocatalysis by High-Throughput Protein Engineering Using Droplet Microfluidics Coupled to Mass Spectrometry

[Image: see text] Directed Evolution is a key technology driving the utility of biocatalysis in pharmaceutical synthesis. Conventional approaches to Directed Evolution are conducted using bacterial cells expressing enzymes in microplates, with catalyzed reactions measured by HPLC, high-performance l...

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Pubblicato in:ACS Omega
Autori principali: Diefenbach, Xue W., Farasat, Iman, Guetschow, Erik D., Welch, Christopher J., Kennedy, Robert T., Sun, Shuwen, Moore, Jeffrey C.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Chemical Society 2018
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6044804/
https://ncbi.nlm.nih.gov/pubmed/30023807
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsomega.7b01973
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