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Enabling Biocatalysis by High-Throughput Protein Engineering Using Droplet Microfluidics Coupled to Mass Spectrometry

[Image: see text] Directed Evolution is a key technology driving the utility of biocatalysis in pharmaceutical synthesis. Conventional approaches to Directed Evolution are conducted using bacterial cells expressing enzymes in microplates, with catalyzed reactions measured by HPLC, high-performance l...

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Detalhes bibliográficos
Publicado no:ACS Omega
Main Authors: Diefenbach, Xue W., Farasat, Iman, Guetschow, Erik D., Welch, Christopher J., Kennedy, Robert T., Sun, Shuwen, Moore, Jeffrey C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2018
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6044804/
https://ncbi.nlm.nih.gov/pubmed/30023807
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsomega.7b01973
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