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Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue

The purpose of this method is to obtain high-integrity RNA samples from enteric ganglia collected from unfixed, freshly-resected human intestinal tissue using laser capture microdissection (LCM). We have identified five steps in the workflow that are crucial for obtaining RNA isolates from enteric g...

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Bibliografske podrobnosti
izdano v:J Vis Exp
Main Authors: May-Zhang, Aaron A., Deal, Karen K, Southard-Smith, E. Michelle
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6042974/
https://ncbi.nlm.nih.gov/pubmed/29985370
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57762
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