Genome-Wide Determination of Gene Essentiality by Transposon Insertion Sequencing in Yeast Pichia pastoris

In many prokaryotes but limited eukaryotic species, the combination of transposon mutagenesis and high-throughput sequencing has greatly accelerated the identification of essential genes. Here we successfully applied this technique to the methylotrophic yeast Pichia pastoris and classified its condi...

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Publicat a:Sci Rep
Autors principals: Zhu, Jinxiang, Gong, Ruiqing, Zhu, Qiaoyun, He, Qiulin, Xu, Ning, Xu, Yichun, Cai, Menghao, Zhou, Xiangshan, Zhang, Yuanxing, Zhou, Mian
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2018
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6033949/
https://ncbi.nlm.nih.gov/pubmed/29976927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-28217-z
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