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Real-time visualization of two-photon fluorescence lifetime imaging microscopy using a wavelength-tunable femtosecond pulsed laser

A fluorescence lifetime imaging microscopy (FLIM) integrated with two-photon excitation technique was developed. A wavelength-tunable femtosecond pulsed laser with nominal pulse repetition rate of 76-MHz was used to acquire FLIM images with a high pixel rate of 3.91 MHz by processing the pulsed two-...

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Bibliografiske detaljer
Udgivet i:Biomed Opt Express
Main Authors: Ryu, Jiheun, Kang, Ungyo, Kim, Jayul, Kim, Hyunjun, Kang, Jue Hyung, Kim, Hyunjin, Sohn, Dae Kyung, Jeong, Jae-heon, Yoo, Hongki, Gweon, Bomi
Format: Artigo
Sprog:Inglês
Udgivet: Optical Society of America 2018
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Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6033550/
https://ncbi.nlm.nih.gov/pubmed/29984109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.9.003449
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