A Structured Workflow for Mapping Human Sin3 Histone Deacetylase Complex Interactions Using Halo-MudPIT Affinity-Purification Mass Spectrometry

Although a variety of affinity purification mass spectrometry (AP-MS) strategies have been used to investigate complex interactions, many of these are susceptible to artifacts because of substantial overexpression of the exogenously expressed bait protein. Here we present a logical and systematic wo...

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Détails bibliographiques
Publié dans:Mol Cell Proteomics
Auteurs principaux: Banks, Charles A. S., Thornton, Janet L., Eubanks, Cassandra G., Adams, Mark K., Miah, Sayem, Boanca, Gina, Liu, Xingyu, Katt, Maria L., Parmely, Tari J., Florens, Laurence, Washburn, Michael P.
Format: Artigo
Langue:Inglês
Publié: The American Society for Biochemistry and Molecular Biology 2018
Sujets:
Technological Innovation and Resources
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6030732/
https://ncbi.nlm.nih.gov/pubmed/29599190
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.TIR118.000661
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