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A Structured Workflow for Mapping Human Sin3 Histone Deacetylase Complex Interactions Using Halo-MudPIT Affinity-Purification Mass Spectrometry
Although a variety of affinity purification mass spectrometry (AP-MS) strategies have been used to investigate complex interactions, many of these are susceptible to artifacts because of substantial overexpression of the exogenously expressed bait protein. Here we present a logical and systematic wo...
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| Publié dans: | Mol Cell Proteomics |
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| Auteurs principaux: | , , , , , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
The American Society for Biochemistry and Molecular Biology
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6030732/ https://ncbi.nlm.nih.gov/pubmed/29599190 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.TIR118.000661 |
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