A Structured Workflow for Mapping Human Sin3 Histone Deacetylase Complex Interactions Using Halo-MudPIT Affinity-Purification Mass Spectrometry

Although a variety of affinity purification mass spectrometry (AP-MS) strategies have been used to investigate complex interactions, many of these are susceptible to artifacts because of substantial overexpression of the exogenously expressed bait protein. Here we present a logical and systematic wo...

Popoln opis

Shranjeno v:
Bibliografske podrobnosti
izdano v:Mol Cell Proteomics
Main Authors: Banks, Charles A. S., Thornton, Janet L., Eubanks, Cassandra G., Adams, Mark K., Miah, Sayem, Boanca, Gina, Liu, Xingyu, Katt, Maria L., Parmely, Tari J., Florens, Laurence, Washburn, Michael P.
Format: Artigo
Jezik:Inglês
Izdano: The American Society for Biochemistry and Molecular Biology 2018
Teme:
Technological Innovation and Resources
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6030732/
https://ncbi.nlm.nih.gov/pubmed/29599190
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.TIR118.000661
Oznake: Označite
Brez oznak, prvi označite!

Podobne knjige/članki