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Promiscuous patching of broken chromosomes in mammalian cells with extrachromosomal DNA

To study double-strand break (DSB)-induced mutations in mammalian chromosomes, we stably transfected thymidine kinase (tk)-deficient mouse fibroblasts with a DNA substrate containing a recognition site for yeast endonuclease I-SceI embedded within a functional tk gene. Cells were then electroporated...

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Bibliografische gegevens
Hoofdauteurs: Lin, Yunfu, Waldman, Alan S.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2001
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC60240/
https://ncbi.nlm.nih.gov/pubmed/11574679
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