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Cell-type-specific brain methylomes profiled via ultralow-input microfluidics

Methylomic analyses typically require substantial amounts of DNA, thus hindering studies involving scarce samples. Here, we show that microfluidic diffusion-based reduced representative bisulfite sequencing (MID-RRBS) permits high-quality methylomic profiling with nanogram-to-single-cell quantities...

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Detalhes bibliográficos
Publicado no:Nat Biomed Eng
Main Authors: Ma, Sai, de la Fuente Revenga, Mario, Sun, Zhixiong, Sun, Chen, Murphy, Travis W., Xie, Hehuang, González-Maeso, Javier, Lu, Chang
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6023403/
https://ncbi.nlm.nih.gov/pubmed/29963329
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