Arg660Ser mutation in Thermus aquaticus DNA polymerase I suppresses T→C transitions: implication of wobble base pair formation at the nucleotide incorporation step

We examined the replication fidelity of an Arg660Ser (R660S) mutant of Thermus aquaticus DNA polymerase I (Taq pol I). In a forward mutation assay, R660S showed a marked reduction in T→C transitions, one of the most frequent errors made by the wild-type enzyme. Steady-state kinetics showed that R660...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Yoshida, Katsushi, Tosaka, Aki, Kamiya, Hiroyuki, Murate, Takashi, Kasai, Hiroshi, Nimura, Yuji, Ogawa, Masanori, Yoshida, Shonen, Suzuki, Motoshi
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2001
Assuntos:	Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC60221/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11600709/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.20.4206
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