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Selection of validated hypervariable regions is crucial in 16S-based microbiota studies of the female genital tract

Next-generation sequencing-based methods are extensively applied in studies of the human microbiota using partial 16 S rRNA gene amplicons. However, they carry drawbacks that are critical to consider when interpreting results, including differences in outcome based on the hypervariable region(s) use...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Graspeuntner, Simon, Loeper, Nathalie, Künzel, Sven, Baines, John F., Rupp, Jan
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6018735/
https://ncbi.nlm.nih.gov/pubmed/29946153
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-27757-8
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