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Purification of Total RNA from DSS-treated Murine Tissue via Lithium Chloride Precipitation
We have developed a protocol to purify RNA from DSS (Dextran Sulfate Sodium)-treated mouse tissues. This method, which prevents downstream inhibition of q-RT-PCR observed in DSS-treated tissues, relies on successive precipitations with lithium chloride.
Shranjeno v:
| izdano v: | Bio Protoc |
|---|---|
| Main Authors: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Bio-Protocol
2018
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6017984/ https://ncbi.nlm.nih.gov/pubmed/29951571 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2829 |
| Oznake: |
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