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New plasma preparation approach to enrich metabolome coverage in untargeted metabolomics: plasma protein bound hydrophobic metabolite release with proteinase K

Plasma untargeted metabolomics is a common method for evaluation of the mechanisms underlying human pathologies and identification of novel biomarkers. The plasma proteins provide the environment for transport of hydrophobic metabolites. The current sample preparation protocol relies on the immediat...

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Publicat a:Sci Rep
Autors principals: Wawrzyniak, Renata, Kosnowska, Anna, Macioszek, Szymon, Bartoszewski, Rafał, Jan Markuszewski, Michał
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6015025/
https://ncbi.nlm.nih.gov/pubmed/29934622
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-27983-0
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