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Characterizing Protein Kinase Substrate Specificity Using the Proteomic Peptide Library (ProPeL) Approach
Characterizing protein kinase substrate specificity motifs represents a powerful step in elucidating kinase-signaling cascades. The protocol described here uses a bacterial system to evaluate kinase specificity motifs in vivo, without the need for radioactive ATP. The human kinase of interest is clo...
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| Publicado no: | Curr Protoc Chem Biol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6014603/ https://ncbi.nlm.nih.gov/pubmed/29927115 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpch.38 |
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