Generation of Recombinant Rotaviruses Expressing Fluorescent Proteins by Using an Optimized Reverse Genetics System

An entirely plasmid-based reverse genetics system for rotaviruses was established very recently. We improved the reverse genetics system to generate recombinant rotavirus by transfecting only 11 cDNA plasmids for its 11 gene segments under the condition of increasing the ratio of the cDNA plasmids f...

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Detalhes bibliográficos
Publicado no:J Virol
Main Authors: Komoto, Satoshi, Fukuda, Saori, Ide, Tomihiko, Ito, Naoto, Sugiyama, Makoto, Yoshikawa, Tetsushi, Murata, Takayuki, Taniguchi, Koki
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2018
Assuntos:
Genome Replication and Regulation of Viral Gene Expression
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6002737/
https://ncbi.nlm.nih.gov/pubmed/29669834
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.00588-18
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