Dynamics of ribosomes and release factors during translation termination in E. coli

Release factors RF1 and RF2 promote hydrolysis of peptidyl-tRNA during translation termination. The GTPase RF3 promotes recycling of RF1 and RF2. Using single molecule FRET and biochemical assays, we show that ribosome termination complexes that carry two factors, RF1–RF3 or RF2–RF3, are dynamic and...

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Bibliografische gegevens
Gepubliceerd in:eLife
Hoofdauteurs: Adio, Sarah, Sharma, Heena, Senyushkina, Tamara, Karki, Prajwal, Maracci, Cristina, Wohlgemuth, Ingo, Holtkamp, Wolf, Peske, Frank, Rodnina, Marina V
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: eLife Sciences Publications, Ltd 2018
Onderwerpen:
Biochemistry and Chemical Biology
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5995542/
https://ncbi.nlm.nih.gov/pubmed/29889659
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.34252
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