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Single-cell RT-PCR in microfluidic droplets with integrated chemical lysis
Droplet microfluidics can identify and sort cells using digital reverse transcription polymerase chain reaction (RT-PCR) signals from individual cells. However, current methods require multiple microfabricated devices for enzymatic cell lysis and PCR reagent addition, making the process complex and...
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| Publicat a: | Anal Chem |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2018
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5991602/ https://ncbi.nlm.nih.gov/pubmed/29256243 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.7b04050 |
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