High-Level Precise Knockin of iPSCs by Simultaneous Reprogramming and Genome Editing of Human Peripheral Blood Mononuclear Cells

We have developed an improved episomal vector system for efficient generation of integration-free induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells. More recently, we reported that the use of an optimized CRISPR-Cas9 system together with a double-cut donor increases homo...

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Detalhes bibliográficos
Publicado no:Stem Cell Reports
Main Authors: Wen, Wei, Cheng, Xinxin, Fu, Yawen, Meng, Feiying, Zhang, Jian-Ping, Zhang, Lu, Li, Xiao-Lan, Yang, Zhixue, Xu, Jing, Zhang, Feng, Botimer, Gary D., Yuan, Weiping, Sun, Changkai, Cheng, Tao, Zhang, Xiao-Bing
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5989814/
https://ncbi.nlm.nih.gov/pubmed/29754960
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2018.04.013
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