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In vitro synthesis of phospholipids with yeast phospholipase B, a phospholipid deacylating enzyme
The gene encoding the Saccharomyces cerevisiae phospholipid deacylation enzyme, phospholipase B (ScPLB1), was successfully expressed in E. coli. The enzyme (Scplb1p) was engineered to have a histidine-tag at the C-terminal end and was purified by metal (Ni) affinity chromatography. Enzymatic propert...
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| Yayımlandı: | Biotechnol Rep (Amst) |
|---|---|
| Asıl Yazarlar: | , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Elsevier
2018
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5989593/ https://ncbi.nlm.nih.gov/pubmed/29876301 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.btre.2018.e00250 |
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