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In vitro synthesis of phospholipids with yeast phospholipase B, a phospholipid deacylating enzyme

The gene encoding the Saccharomyces cerevisiae phospholipid deacylation enzyme, phospholipase B (ScPLB1), was successfully expressed in E. coli. The enzyme (Scplb1p) was engineered to have a histidine-tag at the C-terminal end and was purified by metal (Ni) affinity chromatography. Enzymatic propert...

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Detalhes bibliográficos
Publicado no:Biotechnol Rep (Amst)
Main Authors: Watanabe, Yasuo, Kobayashi, Itsuki, Ohnaka, Takanori, Watanabe, Seiya
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5989593/
https://ncbi.nlm.nih.gov/pubmed/29876301
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.btre.2018.e00250
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