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Correlating transcription initiation and conformational changes by a single subunit RNA polymerase with near base pair resolution

We provide a comprehensive analysis of transcription in real-time by T7 RNA Polymerase (RNAP) using single-molecule fluorescence resonance energy transfer by monitoring the entire life history of transcription initiation by a single RNAP, including stepwise RNA synthesis with near base-pair resoluti...

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Detalhes bibliográficos
Publicado no:Mol Cell
Main Authors: Koh, Hye Ran, Roy, Rahul, Sorokina, Maria, Tang, Guo-Qing, Nandakumar, Divya, Patel, Smita S., Ha, Taekjip
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5983381/
https://ncbi.nlm.nih.gov/pubmed/29775583
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2018.04.018
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