Rapid production of pure recombinant actin isoforms in Pichia pastoris

Actins are major eukaryotic cytoskeletal proteins, and they are involved in many important cell functions, including cell division, cell polarity, wound healing and muscle contraction. Despite obvious drawbacks, muscle actin, which is easily purified, is used extensively for biochemical studies of t...

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Publicado en:J Cell Sci
Main Authors: Hatano, Tomoyuki, Alioto, Salvatore, Roscioli, Emanuele, Palani, Saravanan, Clarke, Scott T., Kamnev, Anton, Hernandez-Fernaud, Juan Ramon, Sivashanmugam, Lavanya, Chapa-y-Lazo, Bernardo, Jones, Alexandra M. E., Robinson, Robert C., Sampath, Karuna, Mishima, Masanori, McAinsh, Andrew D., Goode, Bruce L., Balasubramanian, Mohan K.
Formato: Artigo
Idioma:Inglês
Publicado: The Company of Biologists Ltd 2018
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5976186/
https://ncbi.nlm.nih.gov/pubmed/29535210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/jcs.213827
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