Three dimensional two-photon brain imaging in freely moving mice using a miniature fiber coupled microscope with active axial-scanning

We present a miniature head mounted two-photon fiber-coupled microscope (2P-FCM) for neuronal imaging with active axial focusing enabled using a miniature electrowetting lens. We show three-dimensional two-photon imaging of neuronal structure and record neuronal activity from GCaMP6s fluorescence fr...

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Veröffentlicht in:Sci Rep
Hauptverfasser: Ozbay, Baris N., Futia, Gregory L., Ma, Ming, Bright, Victor M., Gopinath, Juliet T., Hughes, Ethan G., Restrepo, Diego, Gibson, Emily A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2018
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5970169/
https://ncbi.nlm.nih.gov/pubmed/29802371
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-26326-3
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