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Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling

To evaluate the intracellular force transmission between the nucleus and cytoskeleton, we optimized a single cell-based assay that involves the manipulation of living, adherent cells with a fine glass microneedle and a microscope-mounted micromanipulator. The user inserts the microneedle into the cy...

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Détails bibliographiques
Publié dans:Methods Mol Biol
Auteurs principaux: Fedorchak, Gregory, Lammerding, Jan
Format: Artigo
Langue:Inglês
Publié: 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5966280/
https://ncbi.nlm.nih.gov/pubmed/27147047
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-3530-7_16
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