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Reversal of Inhibition by the T7 Concatemer Junction Sequence on Expression From a Downstream T7 Promoter

We have previously reported that placement of the phage T7 concatemer junction (CJ) just upstream of another gene on a plasmid in a T7 system proved to be inhibitory to expression of the downstream gene. We had hypothesized that the inhibition was a result of a readthrough transcript of the CJ eleme...

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Pubblicato in:Gene Expr
Autori principali: Cheng, Li, Goldman, Emanuel
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cognizant Communication Corporation 2018
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5964947/
https://ncbi.nlm.nih.gov/pubmed/11763997
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