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Defining essential elements and genetic interactions of the yeast Lsm2–8 ring and demonstration that essentiality of Lsm2–8 is bypassed via overexpression of U6 snRNA or the U6 snRNP subunit Prp24

A seven-subunit Lsm2–8 protein ring assembles on the U-rich 3′ end of the U6 snRNA. A structure-guided mutational analysis of the Saccharomyces cerevisiae Lsm2–8 ring affords new insights to structure–function relations and genetic interactions of the Lsm subunits. Alanine scanning of 39 amino acids...

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Detalhes bibliográficos
Publicado no:RNA
Main Authors: Roth, Allen J., Shuman, Stewart, Schwer, Beate
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5959253/
https://ncbi.nlm.nih.gov/pubmed/29615482
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.066175.118
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