Immunoprofiling of Chlamydia trachomatis using whole-proteome microarrays generated by on-chip in situ expression

Using Chlamydia trachomatis (Ct) as a complex model organism, we describe a method to generate bacterial whole-proteome microarrays using cell-free, on-chip protein expression. Expression constructs were generated by two successive PCRs directly from bacterial genomic DNA. Bacterial proteins express...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Hufnagel, Katrin, Lueong, Smiths, Willhauck-Fleckenstein, Martina, Hotz-Wagenblatt, Agnes, Miao, Beiping, Bauer, Andrea, Michel, Angelika, Butt, Julia, Pawlita, Michael, Hoheisel, Jörg D., Waterboer, Tim
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5951824/
https://ncbi.nlm.nih.gov/pubmed/29760479
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-25918-3
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