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A rapid and nondestructive protocol for whole-mount bone staining of small fish and Xenopus

Here we propose a new protocol for whole-mount bone staining, which allows the rapid preparation of highly cleared and nondestructive specimens. It only takes 3 days to complete whole procedure for small vertebrates, such as medaka, zebrafish, and Xenopus frogs. In this procedure, we used a newly de...

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Détails bibliographiques
Publié dans:Sci Rep
Auteurs principaux: Sakata-Haga, Hiromi, Uchishiba, Maimi, Shimada, Hiroki, Tsukada, Tsuyoshi, Mitani, Mayumi, Arikawa, Tomohiro, Shoji, Hiroki, Hatta, Toshihisa
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group UK 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5945591/
https://ncbi.nlm.nih.gov/pubmed/29748567
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-25836-4
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