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Inhibition of poly-LacNAc biosynthesis with release of CMP-Neu5Ac feedback inhibition increases the sialylation of recombinant EPO produced in CHO cells

Sialylation of recombinant therapeutic glycoproteins modulates their pharmacokinetic properties by affecting their in vivo half-life. N-glycan branching on glycoproteins increases the number of potential attachment sites for sialic acid. Here, we introduce a new approach for increasing the sialylati...

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Publicat a:Sci Rep
Autors principals: Lee, Chung-Geun, Oh, Myung Jin, Park, Seung-Yeol, An, Hyun Joo, Kim, Jung Hoe
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5940879/
https://ncbi.nlm.nih.gov/pubmed/29740059
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-25580-9
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