Robust Sub-nanomolar Library Preparation for High Throughput Next Generation Sequencing

BACKGROUND: Current library preparation protocols for Illumina HiSeq and MiSeq DNA sequencers require ≥2 nM initial library for subsequent loading of denatured cDNA onto flow cells. Such amounts are not always attainable from samples having a relatively low DNA or RNA input; or those for which a lim...

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Foilsithe in:BMC Genomics
Main Authors: Wu, Wells W., Phue, Je-Nie, Lee, Chun-Ting, Lin, Changyi, Xu, Lai, Wang, Rong, Zhang, Yaqin, Shen, Rong-Fong
Formáid: Artigo
Teanga:Inglês
Foilsithe: BioMed Central 2018
Ábhair:
Methodology Article
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5935984/
https://ncbi.nlm.nih.gov/pubmed/29728062
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-018-4677-y
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