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Imaging Polarization in Budding Yeast
We describe methods for live-cell imaging of yeast cells that we have exploited to image yeast polarity establishment. As a rare event occurring on a fast time-scale, imaging polarization involves a trade-off between spatiotemporal resolution and long-term imaging without excessive phototoxicity. By...
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Publicado no: | Methods Mol Biol |
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Main Authors: | , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2016
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5934585/ https://ncbi.nlm.nih.gov/pubmed/27271891 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-3480-5_2 |
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