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Imaging Polarization in Budding Yeast

We describe methods for live-cell imaging of yeast cells that we have exploited to image yeast polarity establishment. As a rare event occurring on a fast time-scale, imaging polarization involves a trade-off between spatiotemporal resolution and long-term imaging without excessive phototoxicity. By...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: McClure, Allison W., Wu, Chi-Fang, Johnson, Sam A., Lew, Daniel J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5934585/
https://ncbi.nlm.nih.gov/pubmed/27271891
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-3480-5_2
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