Efficient Generation of Transgenic Buffalos (Bubalus bubalis) by Nuclear Transfer of Fetal Fibroblasts Expressing Enhanced Green Fluorescent Protein

The possibility of producing transgenic cloned buffalos by nuclear transfer of fetal fibroblasts expressing enhanced green fluorescent protein (EGFP) was explored in this study. When buffalo fetal fibroblasts (BFFs) isolated from a male buffalo fetus were transfected with pEGFP-N1 (EGFP is driven by...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Lu, Fenghua, Luo, Chan, Li, Nan, Liu, Qingyou, Wei, Yingming, Deng, Haiying, Wang, Xiaoli, Li, Xiangping, Jiang, Jianrong, Deng, Yanfei, Shi, Deshun
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5934360/
https://ncbi.nlm.nih.gov/pubmed/29725050
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-25120-5
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